Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Contributors in Biochemistry

Biochemistry

A form of DNA in which four oligo(G) sequences, either of the same or of different strands, line up either in a parallel, an anti-parallel or in a fold-back (mixed parallel and antiparallel) pattern. ...

Shotgun sequencing

Biology; Biochemistry

An approach to determination of the sequence of a large, randomly selected, piece of genomic DNA. A cosmid that contains the DNA is sonicated into smaller fragments of 1.0-1.5kb, which are ligated ...

Enzymic inverse PCR (EI-PCR)

Biology; Biochemistry

A method for site-directed mutagenesis which generates highly efficient plasmids and which uses only two PCR primers. The primers incorporate the same site for a restriction nuclease, the recognition ...

Van den Bergh reaction

Biology; Biochemistry

A method for colorimetric estimation of serum bilirubin by coupling with diazotized sulphanilic acid. The direct van den Bergh reaction, in aqueous medium, yields the 'direct' bilirubin, i.e. the ...

Transition state

Biology; Biochemistry

In chemical kinetics, the hypothetical state that is mid-way between reactants and products, poised at a point where the reaction is as likely to go forward to products as it is to fall back to ...

Evolutionary tree

Biology; Biochemistry

A reconstruction of the evolutionary relationship of contemporary species that shows their divergence from common ancestors and the branch points at which different species separated. A common ...

Phylogenetic tree

Biology; Biochemistry

A reconstruction of the evolutionary relationship of contemporary species that shows their divergence from common ancestors and the branch points at which different species separated. A common ...