Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Industry: Biology; Biotechnology

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Biochemistry

Sucrose-gradient centrifugation

Biology; Biochemistry

A technique for characterization or preparation of subcellular particles. A swinging-bucket-type centrifuge tube is filled with a sucrose gradient, the bottom of which is most dense and the top least ...

Sensitivity

Biology; Biochemistry

In quantitative terms, the responsiveness of a physiological system to a stimulus, such as an allosteric effector, substrate or hormone. Where X is the magnitude of the stimulus and Y the magnitude ...

Transverse relaxation-optimized spectroscopy (TROSY)

Biology; Biochemistry

A variation on correlation spectroscopy (COSY), the two-dimensional NMR technique for correlation of the chemical shifts of two paramagnetic nuclei, which gives much sharper linewidths than COSY and ...

Double-strand-break repair model

Biology; Biochemistry

Double-strand-break repair model A mechanism proposed for the repair of DNA that has been fragmented at in a non-homologous region. The strands are unwound at the two ends to expose a site of ...

Metal-binding finger

Biology; Biochemistry

A motif of which seven or more may appear in a DNA-binding protein. Each is characterised by two closely spaced cysteine and two histidine residues that serve as ligands for a single Zn 2+ . When ...

Zinc finger

Biology; Biochemistry

A motif of which seven or more may appear in a DNA-binding protein. Each is characterised by two closely spaced cysteine and two histidine residues that serve as ligands for a single Zn 2+ . When ...

Subtractive hybridization

Biology; Biochemistry

A variation of subtractive DNA cloning in which the restriction-nuclease-cleaved tester DNA is biotinylated before annealing with an excess of randomly sheared driver DNA. Annealing is halted at ...