20 Terms
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Biochemistry
The scientific study of the chemistry of living matter.
Industry: Biology; Biotechnology
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Biochemistry
PCR amplification of specific alleles (PASA)
Biology; Biochemistry
PASA; a method for genotyping of single-base mutations. Genomic DNA is used as a template in two reactions, one with a primer that makes a perfect match to the wild-type allele, another with a primer ...
PCR amplification of multiple specific alleles (PAMSA)
Biology; Biochemistry
A method for determination of zygosity. Three primers are used, two of which are allele-specific and one of which matches the complementary strand of both alleles. Allele specificity is achieved by ...
Yeast artificial chromosome (YAC)
Biology; Biochemistry
A cloning vector with a yeast that can accept a relatively large fragment of foreign DNA, up to 1 Mb, in yeast cells; a YAC has telomers on each end and a centromere. Their usefulness is limited by ...
Sticky ended
Biology; Biochemistry
Descriptive of the structure of a double-stranded polynucleotide in which one strand extends further from the end than the other to create a single-stranded 'tail'; especially the product of cleavage ...
Inclusion body
Biology; Biochemistry
An amorphous deposit in the cytoplasm of a cell; an aggregated protein appropriate to the cell but damaged, improperly folded or liganded, or a similarly inappropriately processed foreign protein, ...
Conformational hypothesis
Biology; Biochemistry
An explanation for oxidative phosphorylation that proposes that electron transport builds up a store of energy in the distorted shape of the mitochondrial inner membrane, the relaxation of which is ...
Biosensor
Biology; Biochemistry
A device, especially an electrochemical device that detects, quantitatively and in real time, the presence of an anylate or some biological event (e.g. respiration, enzymic activity, binding to an ...
