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Biochemistry

The scientific study of the chemistry of living matter.

Contributors in Biochemistry

Biochemistry

Self-sustained sequence replication (3SR)

Biology; Biochemistry

A technique for amplification of a DNA sequence, based on the steps of retroviral replication of nucleic acids. Beginning with a clean DNA sequence, DNA-dependent RNA polymerase makes a RNA ...

Affinity cleavage

Biology; Biochemistry

A variation of the footprinting technique in which a DNA molecule is cleaved at a site occupied by a binding protein. A metal chelator (e.g. ethylenediaminetetra-acetate (EDTA)) is covalently ...

Innate immunity

Biology; Biochemistry

A system of blood factors, especially the complement system, and cells, especially macrophages, which affords protection against invading micro-organisms. Unlike acquired or adaptive immunity, which ...

Induced-fit theory

Biology; Biochemistry

A model for interaction of a protein with a ligand in which the protein binds preferentially with one of several conformers of the ligand; especially enzyme action in which the substrate is proposed ...

Nuclear pore complex (NPC)

Biology; Biochemistry

The site on the nuclear membrane through which proteins are transported into, and RNA is transported out of the cell nucleus. The NPC includes proteins that recognise nuclear localization sequences ...

Achilles heel cleavage

Biology; Biochemistry

A method for cleaving of DNA at very specific sequences. The site of cleavage is able to bind a specific protein, e.g. the lac repressor, that already contains a restriction site or is engineered to ...

Rack model

Biology; Biochemistry

A model for interaction of a protein with a ligand in which the protein binds preferentially with one of several conformers of the ligand; especially enzyme action in which the substrate is proposed ...

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