Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Contributors in Biochemistry

Biochemistry

An experimental approach to identification of the gene responsible for a given enzymic activity. Each open reading frame (ORF) of an organism, yeast for instance, is fused to the cDNA of glutathione ...

Diagonal electrophoresis

Biology; Biochemistry

A method for identification of a particular kind of peptide in a mixture by identical electrophoretic steps, the second at a 908 angle to the first, with a chemical modification introduced between ...

Insulator

Biology; Biochemistry

A DNA sequence that contributes to limitation of chromatin activation to distinct segments. In the domain boundary model, the insulator putatively binds nucleoprotein complexes along the chromatin ...

Molar

Biology; Biochemistry

The concentration of 1 mole per litre; 1 mole being Avogadro's number (6.02 x 10 23 ) of molecules, which is equivalent in mass to the sum of the atomic masses (in grams) of the constituent atoms; ...

Entropy effect

Biology; Biochemistry

The acceleration of a reaction that occurs when reactive groups are constrained in a productive orientation, either intermolecularly, as on an enzyme surface, or intramolecularly as in a model ...

Genomic mismatch scanning (GMS)

Biology; Biochemistry

A method to detect and isolate DNA sequences that are candidate genes for inherited disorders for which the gene product is unknown, based on the absence of mismatches in DNA sequences between an ...

Guide RNA (gRNA)

Biology; Biochemistry

A transcript of maxicircle or minicircle DNA, that in lower eukaryotes is thought to function in RNA editing, i.e. by the addition or deletion of U residues at specific sites in the coding regions of ...