Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Industry: Biology; Biotechnology

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Biochemistry

Paracrine

Biology; Biochemistry

Descriptive of a secretion that acts on cells adjacent to the site of its secretion. The term is applied to the non-synaptic neurotransmitter-mediated communication between cells of the central ...

Sanger method (dideoxynucleotide sequencing)

Biology; Biochemistry

A technique for determination of the base sequence of a homogeneous polynucleotide that acts as a template in a replication system. A small amount of a specific dideoxynucleoside triphosphate is ...

A difference cloning method; a technique for comparison of two closely related cell types (e.g. a cell with a deletion mutation and the normal cell), and isolation from one (the tester) of DNA that ...

Circular dichroism (CD)

Biology; Biochemistry

A technique for determination of the asymmetry of a molecule. Unlike in a conventional polarimeter, where light is restricted to oscillation in a plane, in CD the direction of oscillation turns ...

Ribonucleic acid (RNA)

Biology; Biochemistry

A macromolecule formed of repeating riboses linked by phosphodiester bonds between the 3-hydroxyl group of one and the 5-hydroxyl group of the next. A purine, adenine or guanine, or a pyrimidine, ...

Inverse PCR

Biology; Biochemistry

A PCR protocol for amplification of sequences that flank a single known sequence. The source DNA is digested with a restriction endonuclease, circularised and ligated. Primers are constructed that ...

Knock-out

Biology; Biochemistry

An animal or plant from which a certain gene has been deliberately deleted, in order to observe, as a test for the gene's physiological significance, the viability or phenotype of the organism. A ...