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Biochemistry
The scientific study of the chemistry of living matter.
Industry: Biology; Biotechnology
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Biochemistry
Mobile barrier
Biology; Biochemistry
A proposed mechanism of membrane transport. A transmembrane protein, the activated porter, exposes its binding site for the transported molecule to one membrane face and then to the other. In the ...
Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis (SDS/PAGE)
Biology; Biochemistry
A technique to dissociate multimeric proteins and to separate all proteins according to their apparent molecular masses. Proteins are treated with sodium dodecyl sulphate (SDS), an anionic detergent, ...
Bio-imprinting
Biology; Biochemistry
The induction of a new catalytic specificity in an enzyme in an organic solvent. An enzyme and a weakly binding non-substrate/non-product (e.g. chymotrypsin and N-acetyl-D-tryptophan) are suddenly ...
Restriction-site-generating PCR (RG-PCR)
Biology; Biochemistry
A method for detection of a specific mutation that does not itself cause a unique restriction fragment length polymorphism, because it neither creates nor removes a known restriction site. A new ...
Resonance energy transfer
Biology; Biochemistry
A phenomenon in which one fluorophore excites another when there is significant overlap of the emission spectrum of the primary fluorophore with the excitation spectrum of the secondary fluorophore ...
Denaturation
Biology; Biochemistry
The destruction of the ordered folding of a protein or nucleic acid that is required for its normal function. Protein denaturation often involves a change from a specific globular or fibrous ...
Polyclonal antibody
Biology; Biochemistry
A heterogeneous immunoglobulin preparation that contains antibodies directed against one or more determinants on an antigen; the product of daughters of several progenitor cells that have been ...