Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

As contrasted with cutting, the cleaving of a single, or very few, phosphodiester bonds in one polynucleotide strand of a DNA duplex; also the cleavage of a peptide bond in a protein that does not ...

Genomic SELEX

Biology; Biochemistry

A variant of the SELEX (systematic enrichment of ligands by experimental enrichment) procedure, that uses protein ligands and genomic DNA fragments from the same organism to select RNA-protein ...

Positive and negative selection (PNS)

Biology; Biochemistry

A technique to target insertion of a gene at a specific locus in a cell's genome. A plasmid is constructed in which a gene that allows positive selection (e.g. neomycin resistance) is inserted within ...

Immobile DNA junction

Biology; Biochemistry

A branched double-stranded DNA structure, a chi-form in which each of four polynucleotide chains is complementary to and base-paired with the 3'-end of one polynucleotide and the 5'-end of another; ...

Hemiacetal

Biology; Biochemistry

The product of reversible condensation of an aldehyde and an alcohol in which the alcoholic hydroxy group adds across the carbonyl group of the aldehyde, e.g. glucose acting as both an aldehyde and ...

Hemiketal

Biology; Biochemistry

The product of reversible condensation of a ketone and an alcohol in which the alcoholic hydroxy group adds across the carbonyl group of the ketone, e.g. fructose acting as both a ketone and an ...

Triplex DNA

Biology; Biochemistry

Triple-stranded DNA in which the third oligonucleotide strand lies in the major groove of duplex DNA. This strand may also be a synthetic oligonucleotide with a sequence designed to target a specific ...