Molecular biology

Molecular biology

Industry: Biology

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Molecular biology

Oligonucleotide ligation assay (OLA)

Biology; Biochemistry

A method to identify a specific oligonucleotide sequence without use of radiochemicals, electrophoresis or centrifugation. An oligonucleotide biotinylated at its 5'-end and another with a reporter ...

Multiplex PCR

Biology; Biochemistry

A screening technique for any of several mutations known to cluster in a relatively small number of sites (hot spots) in a gene, especially microsatellites. Simultaneous PCR reactions with several ...

Cycle sequencing

Biology; Biochemistry

A method that recruits PCR technology to assist sequencing of small amounts of a single-stranded DNA template. A heat-stable polymerase, a single primer, deoxynucleotides and small amounts of one of ...

Signal hypothesis

Biology; Biochemistry

The proposal that the secretory proteins are initially synthesised with an N-terminal sequence (the signal sequence or leader sequence), that assists it across the membrane of the rough endoplasmic ...

Trans-splicing

Biology; Biochemistry

Discontinuous transcription; the formation of a chimeric mRNA from two transcripts of unlinked loci. trans-Splicing, as distinct from cis-splicing, the more familiar process of excision of introns ...

Light scattering

Biology; Biochemistry

A phenomenon of solutions of macromolecules; used analytically to determine the molecular mass and shape of a macromolecule from the effect on the intensity of scattered light of the wavelength of ...

Protein class

Biology; Biochemistry

A classification according to the content of α-helix and β-pleated sheet. A protein is designated <α if it has a minimum number of residues in an α-helix and fewer than a maximum in β-pleated sheets ...