Molecular biology

Molecular biology

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Molecular biology

Preparative in situ hybridization (prep-ISH)

Biology; Biochemistry

A coincidence cloning technique for isolation of DNA fragments that are characteristic of a small, well characterised portion of a chromosome. Restriction-endonuclease-digested DNA is annealed to ...

Symmetry

Biology; Biochemistry

One of the geometrical properties of a chemical or any other structure. Reflectional symmetry is the property of a molecule or object that permits it to be superimposed on its mirror image; ...

The change in the angle of transmitted plane-polarized light as it passes through a solution of a chiral sample, such that the change is proportional to the concentration of the chiral molecule and ...

Ogston hypothesis

Biology; Biochemistry

The proposal that an enzyme can distinguish in its substrate between two like substituents on a carbon atom that also carries two unlike groups, through binding sites for three of the four ...

Chemical coupling hypothesis

Biology; Biochemistry

Explanation for oxidative phosphorylation by analogy with substrate-level phosphorylation, i.e. when a phosphate derivative of an electron carrier is oxidized, it becomes a high-energy phosphate ...

Prochirality

Biology; Biochemistry

The symmetry characteristic of a molecule, or of a centre within a molecule, by which like groups may be distinguished, e.g. the property of citric acid that allows aconitase to distinguish between ...

Non-competitive inhibition

Biology; Biochemistry

A form of enzyme inhibition in which the inhibitor binds to both the free enzyme and the enzyme-substrate complex, resulting in an increase in K m and a decrease in V max .