Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

The proposal that helper T-cells recognise a foreign material, e.g. a viral antigen, when it is presented on the surface of an antigen-presenting cell as a complex with the major histocompatibility ...

Aggregation

Biology; Biochemistry

The formation of higher-molecular-mass species due to non-covalent adherence of smaller species. Especially for proteins, aggregation is a form of denaturation in which non-polar surfaces of ...

Cassette mutagenesis

Biology; Biochemistry

A method to determine the limits of tolerance of a protein to amino acid substitution. A cloned gene is mutated by synthesis of short segments of the gene with random base substitutions, insertion of ...

Minisatellite variant repeat-specific priming PCR (MVR-PCR)

Biology; Biochemistry

A method for characterization of DNA samples of individuals for forensic purposes, based on sequence variations within the repeating units of variable number tandem repeats (VNTRs). PCR primers are ...

Second law of thermodynamics

Biology; Biochemistry

The relationship of free energy (G), enthalpy (H) and entropy (S) when a system changes from one equilibrium state to another: ΔG=ΔH-TΔS, where T is the absolute temperature. The law provides the ...

RecA-assisted restriction cleavage by endonuclease (RACE cleavage)

Biology; Biochemistry

A method for cleavage of double-stranded DNA at specific restriction sites. A suitable restriction site is selected, around which the base sequence is known, and an oligonucleotide, up to 50 or 60 ...

Differential genome display

Biology; Biochemistry

A method for identifying candidate genes that may determine significant differences between closely related species whose genomes have been mapped, e.g. between a pathogen and a closely related ...