Molecular biology

Molecular biology

Industry: Biology

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Molecular biology

Gel shift assay

Biology; Biochemistry

A method to identify by function a band on a polyacrylamide gel. Electrophoresis is performed on a mixture of proteins before and after treatment with a strong ligand that is specific to one ...

Random DNA amplification (RDA)

Biology; Biochemistry

A method to produce improved strains, e.g. salt tolerance of a plant, by gene amplification. Random genome fragments are introduced into a vector and grown in a bacterium. The plasmid is then ...

Regulation

Biology; Biochemistry

The phenomenon of adjustment of metabolite flux, e.g. glucose that enters the anaerobic glycolysis pathway and exits as lactate, whatever its molecular mechanism. It reflects for each step of the ...

The unequal distribution of a diffusible ion across a semi-permeable membrane when an impermeable electrolyte, such as a protein, is also present on one side; e.g. a solution of an anionic protein ...

Donnan effect

Biology; Biochemistry

G-protein

Biology; Biochemistry

GTP-binding protein; one of a superfamily of proteins that function in, for example, signal transduction (e.g. the G-protein associated with the b-adrenergic receptor), polymerization (e.g. tubulin), ...

Jumping PCR

Biology; Biochemistry

A technique for reconstruction and replication of randomly damaged double-stranded DNA, e.g. that found in mummified tissues or museum samples. In the first PCR cycle each primer is extended until a ...