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Molecular biology
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Molecular biology
Chemical cleavage method
Biology; Biochemistry
A technique for sequence analysis of DNA in which four chemical reactions are applied separately, each to cleave the polynucleotide randomly at one of the four bases. Subsequent polyacrylamide-gel ...
Maxam-Gilbert method
Biology; Biochemistry
A technique for sequence analysis of DNA in which four chemical reactions are applied separately, each to cleave the polynucleotide randomly at one of the four bases. Subsequent polyacrylamide-gel ...
Conjugation
Biology; Biochemistry
In organic chemistry, the interaction of double or triple bonds or aromatic groups, separated by a single bond, which delocalizes their non-bonding electrons; e.g. -CH=CH-CH=CH-, ...
Supercoiling
Biology; Biochemistry
The torsional stress placed on double-stranded DNA, maintained for example by interaction with proteins or by DNA that is circular, and accommodated by a twist imposed on the duplex. A left-handed ...
Instructive theory
Biology; Biochemistry
A now discarded hypothesis that explains antibody specificity by the folding of an antibody structure, which has the potential for many specificities, to the unique surface of a particular antigen.
V(D)J recombination
Biology; Biochemistry
The process of assembling the DNA sequences that encode an immunoglobulin or a T-cell receptor (TCR), the cell-surface protein that recognises an antigen. The process excises polynucleotide sequences ...
Meselson-Radding model
Biology; Biochemistry
A conceptualization of general genetic recombination during meiosis of newly replicated double-stranded DNA. An endonuclease nicks one strand of one daughter duplex; the new 5'-end dissociates and ...
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