Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

A measure of the amphipathicity of an Â¿-helix. Each amino acid side chain is assigned a value, positive or negative, according to its hydrophobicity. These values are used to weight vectors for each ...

Electroblotting

Biology; Biochemistry

An electrophoretic technique for transfer of a protein or nucleic acid from a slab gel, or similar unstable two-dimensional matrix, to a sheet of stable material such as nitrocellulose or ...

Thiocyanate degradation

Biology; Biochemistry

A method for analysis of peptide structure by repetitive sequential degradation from the C-terminus. The free a-carboxyl group is activated with acetic anhydride and reacted with thiocyanate, and the ...

Radioimmunoassay (RIA)

Biology; Biochemistry

A competitive binding assay that depends upon displacement of a radiolabelled ligand from an antibody by the non-labelled standard or test sample, followed by separation of the labelled ligand into ...

Lineweaver-Burk plot

Biology; Biochemistry

A linearized display of kinetic data showing dependence of enzyme activity on substrate concentration, i.e. reciprocal of rate against reciprocal of substrate concentration, therefore also termed a ...

Glycolysis

Biology; Biochemistry

One of the central pathways of metabolism in most eukaryotes and many other cells; the sequence of enzymic reactions that converts glucose into lactic acid (anaerobic glycolysis) or into pyruvate ...

Kinetic assay

Biology; Biochemistry

An enzyme-based assay that measures the amount of substrate present by correlation of the rate of reaction with the known dependence of the rate on substrate concentration, usually under first-order ...