Molecular biology

Molecular biology

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Molecular biology

Van den Bergh reaction

Biology; Biochemistry

A method for colorimetric estimation of serum bilirubin by coupling with diazotized sulphanilic acid. The direct van den Bergh reaction, in aqueous medium, yields the 'direct' bilirubin, i.e. the ...

Transition state

Biology; Biochemistry

In chemical kinetics, the hypothetical state that is mid-way between reactants and products, poised at a point where the reaction is as likely to go forward to products as it is to fall back to ...

Evolutionary tree

Biology; Biochemistry

A reconstruction of the evolutionary relationship of contemporary species that shows their divergence from common ancestors and the branch points at which different species separated. A common ...

Phylogenetic tree

Biology; Biochemistry

A reconstruction of the evolutionary relationship of contemporary species that shows their divergence from common ancestors and the branch points at which different species separated. A common ...

Sucrose-gradient centrifugation

A technique for characterization or preparation of subcellular particles. A swinging-bucket-type centrifuge tube is filled with a sucrose gradient, the bottom of which is most dense and the top least ...

Sensitivity

Biology; Biochemistry

In quantitative terms, the responsiveness of a physiological system to a stimulus, such as an allosteric effector, substrate or hormone. Where X is the magnitude of the stimulus and Y the magnitude ...

Transverse relaxation-optimized spectroscopy (TROSY)

Biology; Biochemistry

A variation on correlation spectroscopy (COSY), the two-dimensional NMR technique for correlation of the chemical shifts of two paramagnetic nuclei, which gives much sharper linewidths than COSY and ...