Molecular biology

Molecular biology

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Molecular biology

Non-productive

Biology; Biochemistry

In enzymology, descriptive of an enzyme-substrate complex other than the Michaelis complex; one that cannot be converted into an enzyme- product complex. In protein chemistry, descriptive of a ...

Control of a metabolic pathway by a metabolite of the pathway that acts in the same direction as the metabolic flux, i.e. downstream or 'later' in the pathway, e.g. the activation of pyruvate kinase ...

X-ray crystallography

Biology; Biochemistry

A method for structural analysis of solids that have a repeating unit; dependent upon the ability of electrons to scatter X-rays, and capable of locating in space atoms larger than hydrogen in ...

Writhing number (W)

Biology; Biochemistry

A topological property of double-stranded DNA; the number of times one double-stranded segment crosses another in circular DNA, commonly referred to as a supercoil; equal to the linking number minus ...

Mobile barrier

Biology; Biochemistry

A proposed mechanism of membrane transport. A transmembrane protein, the activated porter, exposes its binding site for the transported molecule to one membrane face and then to the other. In the ...

Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis (SDS/PAGE)

Biology; Biochemistry

A technique to dissociate multimeric proteins and to separate all proteins according to their apparent molecular masses. Proteins are treated with sodium dodecyl sulphate (SDS), an anionic detergent, ...

Bio-imprinting

Biology; Biochemistry

The induction of a new catalytic specificity in an enzyme in an organic solvent. An enzyme and a weakly binding non-substrate/non-product (e.g. chymotrypsin and N-acetyl-D-tryptophan) are suddenly ...