Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

For solutions in general, the conditions under which thermodynamic properties of a solute are defined, namely 1M. The properties of the solution are those extrapolated from very dilute solutions. In ...

Difference cloning

Biology; Biochemistry

A group of techniques (e.g. subtractive DNA cloning) designed to isolate DNA sequences that are not shared between two DNA sources, e.g. cDNA clones prepared from different tissues, or under ...

Structure-activity relationships by mass spectrometry (SAR by MS)

Biology; Biochemistry

An approach to drug discovery. Sites of interaction of several small molecules with a macromolecule, e.g. aminoglycoside antibiotics with rRNA, are simultaneously probed by MS: the addition to each ...

Tricarboxylic acid cycle (TCA cycle)

Biology; Biochemistry

The metabolic pathway in which acetyl-CoA is catalytically oxidised to carbon dioxide, with the concomitant reduction of NAD + and FAD via a series of tricarboxylic (citric, cisaconitic and ...

Self-sustained sequence replication (3SR)

Biology; Biochemistry

A technique for amplification of a DNA sequence, based on the steps of retroviral replication of nucleic acids. Beginning with a clean DNA sequence, DNA-dependent RNA polymerase makes a RNA ...

Affinity cleavage

Biology; Biochemistry

A variation of the footprinting technique in which a DNA molecule is cleaved at a site occupied by a binding protein. A metal chelator (e.g. ethylenediaminetetra-acetate (EDTA)) is covalently ...

Innate immunity

Biology; Biochemistry

A system of blood factors, especially the complement system, and cells, especially macrophages, which affords protection against invading micro-organisms. Unlike acquired or adaptive immunity, which ...