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Molecular biology
Industry: Biology
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Molecular biology
Induced-fit theory
Biology; Biochemistry
A model for interaction of a protein with a ligand in which the protein binds preferentially with one of several conformers of the ligand; especially enzyme action in which the substrate is proposed ...
Nuclear pore complex (NPC)
Biology; Biochemistry
The site on the nuclear membrane through which proteins are transported into, and RNA is transported out of the cell nucleus. The NPC includes proteins that recognise nuclear localization sequences ...
Achilles heel cleavage
Biology; Biochemistry
A method for cleaving of DNA at very specific sequences. The site of cleavage is able to bind a specific protein, e.g. the lac repressor, that already contains a restriction site or is engineered to ...
Rack model
Biology; Biochemistry
A model for interaction of a protein with a ligand in which the protein binds preferentially with one of several conformers of the ligand; especially enzyme action in which the substrate is proposed ...
Quadruplex DNA
Biology; Biochemistry
A form of DNA in which four oligo(G) sequences, either of the same or of different strands, line up either in a parallel, an anti-parallel or in a fold-back (mixed parallel and antiparallel) pattern. ...
Shotgun sequencing
Biology; Biochemistry
An approach to determination of the sequence of a large, randomly selected, piece of genomic DNA. A cosmid that contains the DNA is sonicated into smaller fragments of 1.0-1.5kb, which are ligated ...
Enzymic inverse PCR (EI-PCR)
Biology; Biochemistry
A method for site-directed mutagenesis which generates highly efficient plasmids and which uses only two PCR primers. The primers incorporate the same site for a restriction nuclease, the recognition ...
