Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

A reporter molecule; a fluorophore-labelled linear polymer that interacts with natural and synthetic phospholipid membranes, and whose fluorophores respond to the polymer ' s physical environment. ...

Enzyme mechanism

Biology; Biochemistry

An elaborate system of nomenclature has been proposed to describe, from the perspective of kinetics, the variety of enzyme mechanisms. Uni, Bi, Ter and Quad refer to the number of substrates or ...

Two-hybrid system

Biology; Biochemistry

An assay for detecting in vivo protein-protein interactions, which depends upon expression of a reporter gene, usually in yeast. As transcription of eukaryotic enzymes are regulated by ...

Framework model

Biology; Biochemistry

A proposed sequence of protein folding events in which the random coil, or denatured form, passes through three stages: the formation of fluctuating embyros, in which disconnected regions of ...

PCR amplification of specific alleles (PASA)

Biology; Biochemistry

PASA; a method for genotyping of single-base mutations. Genomic DNA is used as a template in two reactions, one with a primer that makes a perfect match to the wild-type allele, another with a primer ...

PCR amplification of multiple specific alleles (PAMSA)

Biology; Biochemistry

A method for determination of zygosity. Three primers are used, two of which are allele-specific and one of which matches the complementary strand of both alleles. Allele specificity is achieved by ...

Yeast artificial chromosome (YAC)

Biology; Biochemistry

A cloning vector with a yeast that can accept a relatively large fragment of foreign DNA, up to 1 Mb, in yeast cells; a YAC has telomers on each end and a centromere. Their usefulness is limited by ...