Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

A method for identification of a protein-binding region in a double-stranded DNA fragment. One 5'-end of the DNA fragment is labelled with 32 P and the DNA-fragment-protein complex is treated with a ...

DNase footprinting

Biology; Biochemistry

Infrared spectroscopy

Biology; Biochemistry

IR spectroscopy; a technique that measures absorption of electromagnetic radiation between the long wavelength end of the visible spectrum (780‒800 nm) and the short end of the microwave spectrum ...

Zoo-fluorescence in situ hybridization (zoo-FISH)

Biology; Biochemistry

Cross-species chromosome painting; the comparative mapping of DNA sequences to specific chromosomal loci. Like zoo blotting, zoo-FISH is a method for evaluating evolutionary relationships; it is also ...

Phenol-emulsion reassociation technique (PERT)

Biology; Biochemistry

A method to accelerate several thousand-fold the rate of DNA reassociation. An aqueous solution of single-stranded DNA with a chaotropic salt is shaken with phenol. When the resulting emulsion ...

Atomic absorption analysis

Biology; Biochemistry

A technique for the quantification of small amounts of a metal in solution. Monochromatic light, often generated by excitation of the element in question, passes through a flame in which a test ...

Haworth projection

Biology; Biochemistry

A two-dimensional representation of pyranose and furanose structures in which they are shown as hexagons and pentagons respectively. The lower ring atoms are understood as being towards and the upper ...