Molecular biology

Molecular biology

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Molecular biology

Circular dichroism (CD)

Biology; Biochemistry

A technique for determination of the asymmetry of a molecule. Unlike in a conventional polarimeter, where light is restricted to oscillation in a plane, in CD the direction of oscillation turns ...

Ribonucleic acid (RNA)

Biology; Biochemistry

A macromolecule formed of repeating riboses linked by phosphodiester bonds between the 3-hydroxyl group of one and the 5-hydroxyl group of the next. A purine, adenine or guanine, or a pyrimidine, ...

A PCR protocol for amplification of sequences that flank a single known sequence. The source DNA is digested with a restriction endonuclease, circularised and ligated. Primers are constructed that ...

Knock-out

Biology; Biochemistry

An animal or plant from which a certain gene has been deliberately deleted, in order to observe, as a test for the gene's physiological significance, the viability or phenotype of the organism. A ...

Contig map

Biology; Biochemistry

A characterization of a chromosome or large portion of one by contiguous sequences, which are zones of overlap of partially characterised segments; especially used as a strategy for co-ordination of ...

Propionate rule

Biology; Biochemistry

Analogous to the acetate and isoprene rules, the observation that some natural products are assembled from multiple propionate (propionyl-CoA and methylmalonyl-CoA) units in head-to-tail ...

Cistron

Biology; Biochemistry

A segment of DNA that contains all the information necessary for the production of a single polypeptide and includes both the structural (coding) sequences and regulatory sequences (transcription ...