Molecular biology

Molecular biology

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Molecular biology

Restriction-site-generating PCR (RG-PCR)

Biology; Biochemistry

A method for detection of a specific mutation that does not itself cause a unique restriction fragment length polymorphism, because it neither creates nor removes a known restriction site. A new ...

A phenomenon in which one fluorophore excites another when there is significant overlap of the emission spectrum of the primary fluorophore with the excitation spectrum of the secondary fluorophore ...

Denaturation

Biology; Biochemistry

The destruction of the ordered folding of a protein or nucleic acid that is required for its normal function. Protein denaturation often involves a change from a specific globular or fibrous ...

Polyclonal antibody

Biology; Biochemistry

A heterogeneous immunoglobulin preparation that contains antibodies directed against one or more determinants on an antigen; the product of daughters of several progenitor cells that have been ...

Module

Biology; Biochemistry

In protein chemistry, a building block from which larger proteins are constructed; a recurring folding pattern in proteins, examples of which may have amino acid sequences that are homologous or ...

Iron-sulphur protein

Biology; Biochemistry

One of a class of redox-sensitive proteins and other enzymes characterised by a prosthetic group of one to four iron atoms, depending upon the protein, which are co-ordinated with the sulphur atoms ...

Dideoxynucleotide fingerprinting (ddF)

Biology; Biochemistry

A method for the detection of mutation in a DNA fragment. The DNA serves as a template for replication using a mixture of four normal deoxynucleotides plus one dideoxyribonucleotide. Electrophoresis ...