Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Industry: Biology; Biotechnology

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Biochemistry

Superelectrophile

Biology; Biochemistry

A chemical species with higher than usual electrophilic reactivity, often involving two positively charged centers. Superelectrophiles are intermediates in some enzymatic reactions; e.g. those of ...

Solid-phase sequencing

Biology; Biochemistry

A set of methods for sequencing of proteins and synthesis of proteins and nucleic acids, in which the subject molecule is attached to a solid support to aid in its separation from reagents during ...

The method by which it was demonstrated that proteins are synthesised in the N- to C-terminal direction. A labelled amino acid was supplied to a reticulocyte preparation synthesising haemoglobin and ...

PCR-based differential scanning

Biology; Biochemistry

A method for amplification of a heterogeneous population of cDNAs preparatory to creation of a library. Total mRNA is copied first using an oligo(dT) primer to obtain one copy, then that primer is ...

Synthetase

Biology; Biochemistry

An enzyme, regardless of its mechanism, that couples two substrates by a carbon-carbon, carbon-oxygen, carbon-sulphur or carbon-nitrogen bond that is driven by the hydrolysis of a phosphoanhydride ...

Trans-recognition

Biology; Biochemistry

A refinement of the N-end rule; the determination of the rate of degradation of a cellular multimeric protein composed of different kinds of subunits by amino acid residues of a subunit other than ...

RNA virus

Biology; Biochemistry

A virus with an RNA genome that may be either an mRNA, (+)-RNA, or its complement, (-)-RNA. Class 1 contains (+)-RNA; class 2, (-)-RNA, which is the template for an RNA-dependent RNA polymerase; ...