Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Contributors in Biochemistry

Biochemistry

The dynamic state of microtubules, the addition of subunits at one end, and their removal from the opposite end, which may account for the movement of microtubules in the cell. The nucleation site, ...

Stringency

Biology; Biochemistry

During nucleic acid hybridization or reassociation, the strictness with which the Watson-Crick base-pairing is required under specified conditions of temperature, pH, salt concentration, etc. ...

Dot blotting

Biology; Biochemistry

A method to estimate the concentration of a polynucleotide or protein solution by spotting it on a sheet of nitrocellulose or nylon, hybridising it with a radiolabelled complementary polynucleotide ...

Colony hybridization

Biology; Biochemistry

A technique for screening bacterial colonies for those that contain a desired polynucleotide sequence. A plate is exposed to a labelled oligonucleotide probe with a sequence complementary to part of ...

Antiparallel

Biology; Biochemistry

In protein chemistry, the orientation of extended polypeptide chains that interact in a pleated sheet structure, one chain in an N- to C-terminal direction and the other in a C- to N-terminal ...

Atomic force microscopy (AFM)

Biology; Biochemistry

Also known as scanning force microscopy; a method for mapping the surface of microscopic (e.g. cellular) and even submicroscopic (e.g. macromolecular) surfaces. As a sharp tip passes over the surface ...

Somatic cell nuclear transfer (SCNT)

Biology; Biochemistry

An experimental technique for cloning an organism. Potentially, the technique can be adapted to growing an exactly compatable tissue or organ for transplantation, and would then be called therapeutic ...