Molecular biology

Molecular biology

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Molecular biology

Nernst equation

Biology; Biochemistry

A quantitative expression of the relationship of concentration differences of an ion across a membrane: ΔΨ=(RT/nF)(ln(X n ) e /(X n ) i ), where ΔΨ is the membrane potential, R the gas constant, ...

The introduction of a homologous DNA sequence into a specific site in the genome of a cell, either by replacement of the former sequence, i.e. sequence replacement, or by insertion into the former ...

Cyclic amplification and selection of targets (CASTing)

Biology; Biochemistry

Essentially identical to the selected and amplified (protein) binding site oligonucleotide (SAAB) and target detection assay (TDA) procedures; a procedure for identification of consensus sequences of ...

Representational difference analysis (RDA)

Biology; Biochemistry

A method for isolation and amplification of DNA sequences that differ between two populations (a subtractive technique); e.g. changes associated with antigenic variation in micro-organisms, ...

Differential PCR display

Biology; Biochemistry

A method for identification of mRNAs produced under specific physiological conditions. Total cellular RNA is reverse-transcribed, and the resultant cDNAs are used as templates for PCR. The 3'-primer ...

Quadrapole mass philtre

Biology; Biochemistry

The instrumentation for mass spectroscopy that allows selection of ions of a specific m/e - ratio. As the desorbed material travels down an electric field toward the detector, it passes through a ...

End ligation coincident sequence cloning (EL-CSC)

Biology; Biochemistry

A technique for selection of DNA sequences common to two DNA populations. Of the two populations, the one of lowest complexity (or one made least complex by the creation of representations) is used ...