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Molecular biology
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Molecular biology
Gortner and Grendel model
Biology; Biochemistry
A model in which erythrocyte membranes comprise only phosphoacylglycerols, i.e. no protein or sterol component is envisaged; the model presents a bilayer in which each leaflet is composed of lipids ...
Fluorescence-activated cell sorting (FACS)
Biology; Biochemistry
A technique for separation of cells according to their fluorescence after attachment of a fluorophore to specific cells, e.g. with a fluorescent antibody. Droplets that contain no more than one cell ...
Phase problem
Biology; Biochemistry
In X-ray crystallography, the gap between the requirements for reconstruction of a model - for each reflection (defined by the angle of its diffraction), the amplitude (related to intensity) and ...
Variable number tandem repeat (VNTR)
Biology; Biochemistry
A minisatellite DNA; the repetition of a 35-80bp sequence, to a size of up to 2kbp, that is characteristic of an individual. VNTRs are used in forensic science to identify, or exclude, suspects of a ...
Molecular model
Biology; Biochemistry
A physical representation, e.g. balls and sticks, used to represent the structure of a compound or macromolecule, or a computer graphics simulation of such a structure. More sophisticated versions ...
Rapid amplification of cDNA ends (RACE)
Biology; Biochemistry
A PCR-based method to obtain cDNA clones of rare transcripts which begin with only a partial internal sequence. The 3'- and 5'-ends are analysed separately; a single (-)-strand is first constructed ...
Activation energy
Biology; Biochemistry
The energy needed to raise the reactants, or an enzyme-substrate complex, to the transition state, where it has an equal likelihood of conversion to product or reversion to reactants. This value is ...
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