Molecular biology

Molecular biology

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Molecular biology

Molecular motor

Biology; Biochemistry

A complex of proteins that is responsible for energy, usually ATP hydrolysis-dependent, intracellular movement of an organelle or smaller structure along a cytoskeletal filament; e.g. the ...

A method to construct a site-specific mutated gene and to select it away from the wild-type. A strain of E. coli with only weak dUTPase activity has high levels of dUTP and consequently incorporates ...

Comet assay

Biology; Biochemistry

Single cell gel electrophoresis; a method for assessing DNA damage in individual cells caused, for example, by apoptosis, radiation or chemical mutation. Cells embedded in an agarose gel are ...

Slip-and-click model

Biology; Biochemistry

A molecular explanation of the reversible coiling and uncoliling of bacterial flagella. Each flagellar protofilament is a cylindrical bundle of 11 parallel strands, which may be right-tilted (R) or ...

Conditional expression

Biology; Biochemistry

The technique of constructing cell lines, or transgenic animals, in which the expression of a gene may be controlled by the level of an exogenous compound. One such system, tet-on, involves a plasmid ...

Forced copy-choice model

Biology; Biochemistry

One of two modes of retroviral recombination. In making a DNA transcript of the two genomic RNA strands of a retrovirus, when the polymerase encounters a break on one template strand it continues on ...

Mapping

Biology; Biochemistry

The creation of an outline of locations of genetic markers (genes or other polynucleotide sequences) within the structures of the chromosomes. The methodology determines the resolution of the map. ...