Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

A method for reconstructing the order of random clones in a contig by identification of pairs with overlapping sequences. The DNA clones are deposited as parallel strips on a membrane, then strips of ...

Randomization

Biology; Biochemistry

The migration of an isotope from one position in a labelled metabolite to another position due to the conversion of the metabolite into a symmetrical compound, in which labelled and non-labelled ...

Specificity subsite

Biology; Biochemistry

A part of the active site of a peptidase. Amino acid residues adjacent to the scissile bond are designated P 1 , P 2 , P 3 , etc. on the N-terminal side, and P 1 ', P 2 ', P 3 ', etc. on the ...

Solvent isotope effect

Biology; Biochemistry

The effect on an enzyme's kinetics of inclusion of 2H 2 O; often expressed as D k, the ratio of the reaction rate constant in H 2 O to the rate constant in 2H 2 O. An extension of this approach is a ...

Ligation-mediated PCR

Biology; Biochemistry

A PCR technique, similar to anchored PCR, applied to double-stranded DNA molecules for which the sequence of only one end is known, e.g. in in vivo footprint analysis to detect sites protected by a ...

Primer extension analysis

Biology; Biochemistry

A method for measurement of the amount of a specific mRNA and its length relative to a known sequence. A labelled synthetic primer designed for complementarity to a sequence in the mRNA, usually ...

2-D crystallography

Biology; Biochemistry

A technique for low-resolution (10Â20 Ã) determination of shapes of proteins, especially large, complex proteins that are not amenable to crystallisation by conventional techniques. A monomolecular ...