Molecular biology

Molecular biology

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Molecular biology

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Biology; Biochemistry

A secondary nucleotide sequence specificity of an endonuclease. After the canonical specificity is established, a library of potential substrates may be created by substituting at each sequence ...

Transesterification

Biology; Biochemistry

The substitution of one alcohol for another in an ester bond, e.g. the displacement of one 3'-hydroxy group of a phosphodiester bond by the 3'-hydroxy group of another nucleotide during the ...

Descriptive of the folding of a protein upon itself in several convolutions to create interactions of the side chains in many weak salt bridges, hydrogen bonds and hydrophobic bonds that result in a ...

Thick filament

Biology; Biochemistry

In muscle cells, an aggregate of myosin molecules in which the long, fibrous tails are intertwined in a superhelix that leaves the globular heads as knobs in a helical array over the surface. The ...

Treadmilling

Biology; Biochemistry

The dynamic state of microtubules, the addition of subunits at one end, and their removal from the opposite end, which may account for the movement of microtubules in the cell. The nucleation site, ...

Stringency

Biology; Biochemistry

During nucleic acid hybridization or reassociation, the strictness with which the Watson-Crick base-pairing is required under specified conditions of temperature, pH, salt concentration, etc. ...

Dot blotting

Biology; Biochemistry

A method to estimate the concentration of a polynucleotide or protein solution by spotting it on a sheet of nitrocellulose or nylon, hybridising it with a radiolabelled complementary polynucleotide ...