Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

A technique for screening bacterial colonies for those that contain a desired polynucleotide sequence. A plate is exposed to a labelled oligonucleotide probe with a sequence complementary to part of ...

Antiparallel

Biology; Biochemistry

In protein chemistry, the orientation of extended polypeptide chains that interact in a pleated sheet structure, one chain in an N- to C-terminal direction and the other in a C- to N-terminal ...

Atomic force microscopy (AFM)

Biology; Biochemistry

Also known as scanning force microscopy; a method for mapping the surface of microscopic (e.g. cellular) and even submicroscopic (e.g. macromolecular) surfaces. As a sharp tip passes over the surface ...

Somatic cell nuclear transfer (SCNT)

Biology; Biochemistry

An experimental technique for cloning an organism. Potentially, the technique can be adapted to growing an exactly compatable tissue or organ for transplantation, and would then be called therapeutic ...

Superelectrophile

Biology; Biochemistry

A chemical species with higher than usual electrophilic reactivity, often involving two positively charged centers. Superelectrophiles are intermediates in some enzymatic reactions; e.g. those of ...

Solid-phase sequencing

Biology; Biochemistry

A set of methods for sequencing of proteins and synthesis of proteins and nucleic acids, in which the subject molecule is attached to a solid support to aid in its separation from reagents during ...

Dintzis experiment

Biology; Biochemistry

The method by which it was demonstrated that proteins are synthesised in the N- to C-terminal direction. A labelled amino acid was supplied to a reticulocyte preparation synthesising haemoglobin and ...